Figure 1From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tardaSurvival curve of zebrafish embryos treated by immersion in E. tarda suspension. For each of the independent 7 experiments 20 embryos at 25 hpf were immersed for 5 h in 108 CFUs/ml of E. tarda or in clean egg water as a control. Subsequently embryos were washed and transferred to fresh egg water, and survival was monitored for 4 days. In one experiment, heat-killed bacteria (45 min at 95°C) were included as an extra control group. Survival varied between approximately 25 and 75%.Back to article page