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Figure 3 | BMC Immunology

Figure 3

From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Figure 3

Time course analysis of marker gene expression in embryos treated by immersion in E. tarda suspension. Embryos were immersed at 25 hpf for 5 h in E. tarda suspension or in clean egg water as a control. Subsequently, embryos were washed and transferred to fresh egg water. RNA was isolated from pools of 20 embryos at 5, 24 and 48 h after the start of exposure (hpe) and the expression levels of cyp1a (a), irg1l (b), mmp9 (c), and il1b (d) were quantified by qPCR. A representative example of three independent experiments is shown. Relative induction levels are shown with the lowest expression level set at 1.

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