Figure 3From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tardaTime course analysis of marker gene expression in embryos treated by immersion in E. tarda suspension. Embryos were immersed at 25 hpf for 5 h in E. tarda suspension or in clean egg water as a control. Subsequently, embryos were washed and transferred to fresh egg water. RNA was isolated from pools of 20 embryos at 5, 24 and 48 h after the start of exposure (hpe) and the expression levels of cyp1a (a), irg1l (b), mmp9 (c), and il1b (d) were quantified by qPCR. A representative example of three independent experiments is shown. Relative induction levels are shown with the lowest expression level set at 1.Back to article page