Skip to main content
Figure 4 | BMC Immunology

Figure 4

From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Figure 4

Marker gene expression in immersion tests after fractionation of E. tarda suspension. The E. tarda suspension as used for the immersion experiments in Figure 1-3 was separated into two fractions by centrifugation. Embryos were immersed at 25 hpf for 5 h in the wash fluid obtained after centrifugation, or in the resuspended bacterial pellet (washed bacteria), or in clean egg water as a control. Subsequently, embryos were washed and transferred to fresh egg water. RNA was isolated from pools of 20 embryos at 5 and 24 h after the start of exposure (hpe) and the expression levels of cyp1a (a), irg1l (b), mmp9 (c), and il1b (d) were quantified by qPCR. A representative example of two independent experiments is shown. Relative induction levels are shown with the lowest expression level set at 1.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us