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Figure 5 | BMC Immunology

Figure 5

From: Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Figure 5

Marker gene expression in individual embryos treated by immersion in E. tarda suspension. Pools of 20 embryos were immersed at 25 hpf for 5h in E. tarda suspension or in clean egg water as a control. Subsequently, embryos were washed and transferred to fresh egg water. RNA was isolated from 5 single embryos at 48 h after the start of exposure (hpe) and expression levels of cyp1a (a), irg1l (b), mmp9 (c) and il1b (d) were measured by qPCR. Relative induction levels are shown with the lowest expression level set at 1.

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BMC Immunology

ISSN: 1471-2172

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