Endogenous annexin A1 counter-regulates bleomycin-induced lung fibrosis

BMC Immunology

Table 1 Annexin-A1 protein expression in wild type mouse lung tissue and leukocytes during the process of fibrosis induced by bleomycin

Wild type	Epithelial cells	PMN			MPC
		Intra-vascular	Trans-migrated	Intra-alveolar	Intra-vascular	Tissue	Intra-alveolar
Sham	45 ± 2	101 ± 7	62 ± 2	80 ± 8	41 ± 3	43 ± 4	72 ± 4
Bleo 7d	66 ± 3 *	63 ± 3 *	83 ± 2 *	82 ± 7	63 ± 2 *	72 ± 4 *	74 ± 5
Bleo 7d + Ac2-26	70 ± 5 *	62 ± 2 *	62 ± 3 #	73 ± 8	45 ± 3 #	74 ± 5 *	73 ± 4
Bleo 21d	48 ± 2	64 ± 3 *	70 ± 4	70 ± 7	42 ± 1	44 ± 4	43 ± 4 *
Bleo 21d + Ac2-26 daily	60 ± 3 *#	110 ± 9 #	86 ± 5 *	79 ± 8	55 ± 3	44 ± 3	104 ± 6 #
Bleo 21d + Ac2-26 14-21d	70 ± 4 *#	70 ± 3 *	91 ± 7 *#	81 ± 7	45 ± 4	50 ± 2	55 ± 1

AnxA1 protein expression was analyzed, as described in Material and Methods, in wild type mice lung at 0, 7 and 21 days post-bleomycin i.t. administration. Also a group of mice were daily i.p. treated or post-treated with the peptide Ac2-26 as described in Material and Methods section. Values (densitometric units) are mean ± SEM of 10 histological tissue sections analyzed from five mice per group. * P < 0.05 versus sham group values. # P < 0.05 versus corresponding control group values. PMN, polymorphonuclear cells; MPC, monocyte/macrophages.

ISSN: 1471-2172