Figure 3

T cells are activated in the CNS of WNV-inoculated mice. Adult, female B6 mice were inoculated SC with 10³ PFU of WNV in the left rear footpad. At various times post-inoculation, four WNV-inoculated mice were sacrificed and perfused with perfusion buffer. CNS mononuclear cells were collected, and flow cytometry was performed for various cell markers. Cells were gated on the CD45⁺ population as shown in Figure 1. (A) Representative scatter plots are shown for activation markers, CD69 or CD25, on cells gated for the CD4⁺ or CD8⁺ population from WNV-inoculated mice. The percentage of (B) CD8⁺ T cells and (C) CD4⁺ T cells expressing either CD69 or CD25 in the brains of WNV-inoculated mice are shown at various times post-inoculation. Bars and error bars represent the average and SD of four mice.