Cytokine production by unimmunized and OVA/Alum immunized D011.10xRAG2-/- mice. Splenocytes isolated from either naïve (unimmunized) or OVA/Alum immunized D011.10xRAG2-/- mice were cultured in media containing 20 U/ml IL-2 and were either stimulated with PMA/Ionomycin (PMA/Ion) or left untreated for 18 h. IL-4 and IL-5 secretion into the cell culture supernatant was quantitated using ELISA. Data represented as concentration of cytokine ± SEM in ng/ml. n = 3 for each group. * p < 0.05.