Degree of BAL eosinopilia, cytokine and chemokine secretion in RAG2-/- STAT6xRAG2-/- or IL-4RαxRAG2-/- mice. The asthma protocol used in this study is depicted in (A). In vivo primed DO11.10+ CD4+ T cells were adoptively transferred into RAG2-/-, STAT6xRAG2-/- or IL-4RαxRAG2-/- mice. Mice were primed with either alum or 100 μg of Ova in alum i.p on d. 1 & 6 and then challenged with nebulized PBS or 1% Ova in PBS on d. 12 &14. BAL fluid was recovered 48 h after the last challenge and cells were analyzed by differential staining. Lung tissue was collected for histological analysis. (B) The total number of cells (T), macrophages (M), eosinophils (E), lymphocytes (L) and polymorphoneutrophils (P) present in the BAL in RAG2-/-, STAT6xRAG2-/- and IL-4RαxRAG2-/- mice are represented here in the form of bar graphs. Data represented as numbers ± SEM. * (p < 0.05), represents statistically significant differences between the OVA and Alum treated mice in each group. n = 5 for Ova treated mice, n = 3 for alum treated. (C) Chemokine and cytokine levels in BAL samples from OVA primed and challenged RAG2-/-, STAT6xRAG2-/- and IL-4RαxRAG2-/- mice were analyzed using a multiplex array system. Data are presented as mean chemokine or cytokine level in pg/ml ± SEM. * p < 0.05; ** p < 0.01; + p < 0.0001. n = 4 for RAG2-/- mice, n = 3 each for STAT6xRAG2-/- or IL-4RαxRAG2-/- mice. Representative data from one of three independent experiments is shown.