Figure 3
Systemic cytokine and chemokine profiles in NZB/WF1 mice immunized with phthalate-KLH. NZB/WF1 mice (N = 5) were immunized as described under Materials and Methods, and sera (collected after 2nd booster, and then the 3rd booster with the antigen phthalate-KLH alone) were diluted 1:5 and subjected to cytokine and chemokine antibody arrays. Each cytokine was measured in duplicate spots and displayed in the array template as shown. The image shown in (A) represents serum cytokine and chemokine profiles of mice treated with different adjuvants in combination with antigen after receiving two booster immunizations, and the image shown in (B) represents serum cytokine and chemokine profiles of mice treated with different adjuvants in combination with the antigen following the 3rd booster immunization with antigen alone.