Flow cytometric analysis of human peripheral blood CD4+ T cells. The expression of CD300a/c on central (TCM) and effector memory (TEM) CD4+ T cells was determined with three staining strategies: CD45RO and CD62L (upper panels), CD45RO and CD27 (middle panels) and CD45RA and CCR7 (lower panels). A representative donor is shown for each staining. Bar graphs represent the average ± SEM of the percentage of CD300a/c+ cells within TCM and TEM. Results shown are from 12 healthy donors.