Figure 4
From: Differential expression of CD300a/c on human TH1 and TH17 cells
IL-17a single producing cells tend to be CD300a/c-. Purified CD4+ T cells were stimulated with PMA and ionomycin for 4-5 h in the presence of GolgiStop (monensin). Then, cells were stained for cell surface expression of CD300a/c and intracellular production of IL-17a and other cytokines. A representative donor is shown for (A) IL-17a, for TNF-α and IFN-γ producing cells, (B) IL-17a, IL-2 and IFN-γ producing cells, and for (C) IL-17a, IL-2 and TNF-α producing cells. Graph bars represent the average ± SEM of the percentage of CD300a/c+ cells within the cytokine producing cells. Results are from 8-12 healthy donors.