MyD88-deficiency did not impair arginase-1, RELMα, and Ym1 expression in BMMϕs. mRNA was extracted and real-time RT-PCR for arginase-1, RELMα, and Ym1 expression was carried out to examine AAMϕ generation. The black bars represent the mRNA expression in mice treated with IL-4. The mRNA expression is shown as a percent of the positive control sample and β-actin was used to normalize the experiments. Results are compiled from three independent experiments.