Effect of ketoprofen on single leukocyte migration: A-B: Leukocyte migration was studied using digital time-lapse microscopy. Ketoprofen caused a dose-dependent inhibition of leukocyte speed (A) which partially recovered after the removal of ketoprofen (B). C: Representative images of migrating leukocytes in collagen matrix at baseline, leukocyte depolarization 5 min after application of 2 mM of ketoprofen and partial restitution of leukocyte migration after removal of ketoprofen. D-E: Blockade of activated leukocytes using topic application of ketoprofen in vivo: quantitative analysis of leukocyte speed (D) and representative image of intravital microscopy (E). Application of ketoprofen gel 60 min after IL-8 injection caused a rapid leukocyte depolarization and movement stop (leukocytes are showed as green-fluorescent cells).