Two-dimensional gel electrophoresis of lung tissue samples obtained from wild type (WT) and VIP KO (KO) mice. The above are typical images obtained from gels of both tissue types. The staining intensities of the protein spots were analyzed using BioRad PDQuest software. The three spots (3204, 3304 and 6304) in the WT gel were significantly and substantially under-expressed in the KO gel. The three spots (7105, 7205 and 8101) in the KO gel were significantly and substantially over-express in the KO gel. Spot 9206, encircled in both gels was expresses at essentially equal levels in both gels.