Vasoactive Intestinal Peptide Knockout (VIP KO) mouse model of sulfite-sensitive asthma: up-regulation of novel lung carbonyl reductase

Table 1 Summary of SSP optical density and LC/MS/MS determined protein identity of the excised spots

SSP#	Protein	Symbol	IPI Accession #	spectra	peptides	MW(kDa)/pI calculated	MW(kDa)/pI observed^a	Av^c KO OD	Av WT OD	KO/WT	WT/KO	p value (t-test)
7105	Triosephosphate Isomerase	Tpi1	00467833	8	4	26.71/6.9	25.6/7.4	39301	0	∞	0	0.017
7205	Dihydropteridine Reductase	Qdpr	00459279	5	3	25.57/7.7	27.1/7.5	222665	0	∞	0	0.032
8101	Carbonyl Reductase	Cbr2	00128642	15	7	25.96/9.1	25.8/7.8	210771	2855	73.8	0.01	0.044
3304	Pyruvate Dehydrogenase	Pdhb	00132042	36	13	38.94/6.4	37.1/5.6	72099	312442	0.23	4.33	0.011
3204	Superoxide Dismutase	Sod3	00114319	12	4	27.39/6.4	32.8/5.6	10569	90285	0.12	8.54	0.002
6304	Glycerol-3-Phosphate Dehydrogenase	Gpd1	00230185	16	8	37.57/6.8	36.6/6.9	19546	148182	0.13	7.58	0.038
9206	Carbonyl Reductase	Cbr2	00128642	33	16	25.96/9.1	25.96/9.1^b	546335	521831	1.05	0.96	0.905

^a Based on internal benchmarks.
^b Served as one of four internal benchmarks.
^c Average optical density of spots from four gels.
KO/WT and WT/KO are ratios of average optical densities and the t-Test p-values are two-tailed, unpaired determinations from four gels of each type.

ISSN: 1471-2172