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Table 1 Summary of SSP optical density and LC/MS/MS determined protein identity of the excised spots

From: Vasoactive Intestinal Peptide Knockout (VIP KO) mouse model of sulfite-sensitive asthma: up-regulation of novel lung carbonyl reductase

SSP# Protein Symbol IPI Accession # spectra peptides MW(kDa)/pI calculated MW(kDa)/pI observeda Avc KO OD Av WT OD KO/WT WT/KO p value (t-test)
7105 Triosephosphate Isomerase Tpi1 00467833 8 4 26.71/6.9 25.6/7.4 39301 0 0 0.017
7205 Dihydropteridine Reductase Qdpr 00459279 5 3 25.57/7.7 27.1/7.5 222665 0 0 0.032
8101 Carbonyl Reductase Cbr2 00128642 15 7 25.96/9.1 25.8/7.8 210771 2855 73.8 0.01 0.044
3304 Pyruvate Dehydrogenase Pdhb 00132042 36 13 38.94/6.4 37.1/5.6 72099 312442 0.23 4.33 0.011
3204 Superoxide Dismutase Sod3 00114319 12 4 27.39/6.4 32.8/5.6 10569 90285 0.12 8.54 0.002
6304 Glycerol-3-Phosphate Dehydrogenase Gpd1 00230185 16 8 37.57/6.8 36.6/6.9 19546 148182 0.13 7.58 0.038
9206 Carbonyl Reductase Cbr2 00128642 33 16 25.96/9.1 25.96/9.1b 546335 521831 1.05 0.96 0.905
  1. a Based on internal benchmarks.
  2. b Served as one of four internal benchmarks.
  3. c Average optical density of spots from four gels.
  4. KO/WT and WT/KO are ratios of average optical densities and the t-Test p-values are two-tailed, unpaired determinations from four gels of each type.