Figure 3

Accumulation of effector/memory CD4⁺ cells in transgenic (TgL) strains of mice. The percentages of CD44^highCD62L^lowCD25^-CD4⁺ T cells (effector/memory CD4⁺T cells) in a total of CD4⁺T cells are shown in the dot grams; (A) spleen, (C) lung. The absolute numbers of effector/memory CD4⁺ T cells are shown in the bar grams; (B) spleen, (D) lung. The absolute number was calculated from the percentage of this subset and the total cell number in each organ. The results represent a mean ± SD of 6-8 mice per each group. The results of flow cytometry (A and C) are representative of three independent experiments performed using 8 to 10 week-old mice. The significant difference between the two groups was evaluated by two tailed t- test. *, p < 0.01; **, p < 0.001.