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Figure 1 | BMC Immunology

Figure 1

From: The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function

Figure 1

T cell-specific deletion of EFNB1 in Lck-EFNB1f/f mice according to immunofluorescent microscopy and flow cytometry. A. Thymocytes from EFNB1f/f (upper row) or Lck-EFNB1f/f (bottom row) mice were stained with FITC-rat anti-Thy1.2 (green) and PE-rat anti-EFNB1 (red) mAbs. Micrographs of the cells with pseudocolouring are shown. B. CD3-gated spleen T cells from EFNB1f/f/cre mice or EFNB1f/f controls were analyzed for EFNB1 expression by flow cytometry. The dotted line represents isotypic control Ab, and solid line, anti-EFNB1 Abs. Percentage of EFNB1 positive cells was indicated. The experiments were repeated three times and data from representative ones are shown.

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