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Figure 5 | BMC Immunology

Figure 5

From: The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function

Figure 5

Normal activation and proliferation of Lck-EFNB1f/f T cells. A. Activation marker expression. Total spleen cells from Lck-EFNB1f/f and EFNB1f/f mice were stimulated with soluble anti-CD3 mAb and anti-CD28 mAbs for 48 hours, and stained with PE-rat anti-mouse Thy1.2 and FITC-rat anti-mouse CD25 or CD69, followed by flow cytometry analysis. B. EFNB1 expression in activated T cells. Total spleen cells from EFNB1 f/f mice were cultured in medium or with anti-CD3 mAb for 48 hours, and stained with Thy1.2 -FITC and PE-rat anti-EFNB1 mAbs. The percentage of EFNB1 positive cells gated on T cells is indicated. C and D. T cell proliferation. Purified T cells from Lck-EFNB1f/f and EFNB1f/f mice were cultured in wells coated with solid phase anti-CD3 (C) or anti-CD3 plus anti-CD28 mAb (D) for 24 to 72 h. The cells were pulsed with3 H-thymidine for the last 16 h of the culture. Samples were in triplicate. Mean ± SD of cpm are shown. Experiments were repeated more than 5 times and representative data are shown.

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