Impact of acute stress on antimicrobial polypeptides mRNA copy number in several tissues of marine sea bass (Dicentrarchus labrax)

Table 2 Sequences of the primers used for molecular cloning.

Primer name	5'→3' sequence	Purpose
Hemoglobin FW1	ATGGTCCAGTGGTCAGATGC	RT-PCR
Hemoglobin RV1	CTTCAGGCTTCTTGCTGAATG
Hemoglobin FW2	TGGACAGGGCTGTGAAGAAC
Hemoglobin RV2	AAGTTCCTGGCTGTGGTCGT
HLP1 FW1	AAGAAGGGCTCCAAGAAAGC
HLP1 RV1	GCACTAAAGCGGTGACGAA
HLP1 RV2	ACCATCACCTCCAGGGAGAT
HLP1 RV3	GGCTAAACACGCAGTGTC
HLP1 RV4	GGCTAAGCACGCCGTGTC
HLP2 FW1	CCAAAGCGCCCAAGAAGA
HLP2 RV1	TAAAGGCATCCTGCTGCAG
HLP2 FW2	TGTCTCTCGCTGCTCTGAAG
HLP2 RV2	CAAGAAGGCCAAGAAACCC
T3 Hb-LP sense	caattaaccctcactaaaggga ATGGTCCAGTGGTCAGATGC	Standard curve
Hb-LP antisense	AAGTTCCTGGCTGTGGTCGT
T3 HLP1 sense	caattaaccctcactaaaggga AAGAAGGGCTCCAAGAAAGC
HLP1 antisense	ACCATCACCTCCAGGGAGAT
T3 HLP2 sense	caattaaccctcactaaaggga CCAAAGCGCCCAAGAAGA
HLP2 antisense	CAAGAAGGCCAAGAAACCC
T7 Dicentracin sense	gtaatacgactcactataggg AGTGCGCCACGCTCTTTC
Dicentracin antisense	CTAGTCAAAAGCTGCGCGCT
Hb-LP Forward	GGTCCAGTGGTCAGATGCA	Real time RT-PCR
Hb-LP Reverse	ACGATCAGAAGTCTGGTCAAAGC
Hb-LP Taqman^® probe	CCGCCATCACAAGCTGG
HLP1 Forward	CGTGTCTAAGGTCACCAAGA
HLP1 Reverse	GTACACGTAGATGGCGTAGCT
HLP1 Taqman^® probe	CCGGCAAGAAGAAGAG
HLP2 Forward	CCCAAGAAGAGAGCCAAGTCT
HLP2 Reverse	CTGATACTGAAGGCCGCGT
HLP2 Taqman^® probe	AAGAAGAAGACGGGTCCCTC
Dicentracin Forward	TGCGCCACGCTCTTTCTT
Dicentracin Reverse	CCCCAGGTTCAGCCATGAG
Dicentracin Taqman^® probe	ACGACCATCGACAGCAC

ISSN: 1471-2172