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Table 3 Characteristics of the in vitro-transcribed AMPP mRNAs

From: Impact of acute stress on antimicrobial polypeptides mRNA copy number in several tissues of marine sea bass (Dicentrarchus labrax)

mRNA

Nucleotides

MW

ng/μl

No. molecules/μl

 

A

U

G

C

   

Hb-LP

104

92

116

104

134350.2

349

1.56 × 1012

HLP1

69

44

64

71

80108.6

134

1.01 × 1012

HLP2

133

45

125

115

135969.6

271

4.68 × 1014

Dicentracin

57

53

63

66

77042.8

605.5

4.73 × 1012

  1. (A: adenine; U: uracil; G: guanine; C: cytosine; MW: molecular weight). Concentration (ng/μl) was calculated by using the value of absorbance at 260 nm.