Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice

BMC Immunology

Table 1 Ab production from B6 and B6.TC FOB and MZB cells in response to various stimuli in 5 d cultures

	B6 FO		TC FO		B6 MZ		TC MZ
	mean	SD	mean	SD	mean	SD	mean	SD
	total IgM
control	0.09	0.02	0.10	0.01	0.08	0.01	0.12**	0.01
LPS	0.57	0.02	0.67	0.09	0.68	0.17	1.31***	0.06
CpG	0.26	0.03	0.59**	0.01	0.57	0.01	0.79*	0.05
protein ICs	0.08	0.00	0.21**	0.03	0.11	0.01	0.63***	0.01
chromatin ICs	0.16	0.01	0.21	0.00	0.15	0.12	0.37*	0.01
anti-CD40	0.11	0.03	0.11	0.04	0.32	0.02	0.95***	0.08
B6 CD4	0.19	0.02	0.19	0.03	0.22	0.05	0.24	0.01
TC CD4	0.31	0.05	0.30	0.02	0.35	0.03	0.37	0.04
	ssDNA IgM
control	0.05	0.00	0.07	0.02	0.06	0.01	0.06	0.01
LPS	0.40	0.09	0.55*	0.01	0.70	0.04	0.79	0.07
anti-CD40	0.06	0.01	0.06	0.01	0.10	0.03	0.25**	0.07
	dsDNA IgM
control	0.05	0.00	0.06	0.01	0.05	0.00	0.06	0.00
LPS	0.17	0.06	0.20	0.04	0.33	0.04	0.35	0.08

Mean ODs and standard deviations (SD) of ELISA assays performed on supernatants diluted 1:10 from one representative experiment.
* indicates the level of significance of t tests between B6 and B6.TC FOB cells on the left or between B6 and B6.TC MZB cells on the right. *: p < 0.05; **: p < 0.01, ***: p < 0.001.

ISSN: 1471-2172