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Figure 2 | BMC Immunology

Figure 2

From: Neuroantigen-specific, tolerogenic vaccines: GM-CSF is a fusion partner that facilitates tolerance rather than immunity to dominant self-epitopes of myelin in murine models of experimental autoimmune encephalomyelitis (EAE)

Figure 2

The GM-CSF domain of murine GMCSF-MOG potentiated the antigenic recognition of MOG35-55 by rat T cells. (A) The rat T cell clone RsL.11 or the rat 1E2 or 1B3 T cell hybrids were cultured with Lewis rat splenic APC and designated concentrations (x-axis) of the rat GMCSF-(GP69-88) fusion protein or the GP69-88 synthetic peptide. Supernatants were collected after 24 hrs of culture and assayed for IL-2 production. (B) A rat MOG(35-55)-specific T cell line was cultured with rat splenic APC and designated concentrations of murine GMCSF-MOG, the synthetic MOG35-55 peptide, or the IgV extracellular domain of rat or macaca MOG. Cultures were pulsed with 1 uCi of [3H]thymidine during the last 24 hours of a 3-day culture. These data are representative of four experiments.

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BMC Immunology

ISSN: 1471-2172

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