Figure 4

Induction of MOG-specific tolerance required covalent linkage of the GM-CSF and MOG domains. C57BL/6 mice were treated with GMCSF-MOG, a mixture of murine GM-CSF and MOG35-55, the MOG35-55 peptide, GM-CSF, or saline subcutaneously on days -21, -14, and -7 (n = 8, all groups, 2 nanomoles per dose). Mice were then immunized on day 0 with 200 ug MOG35-55 in CFA plus Pertussis toxin (200 ng i.p.) on days 0 and 2. Table 2 and Figure 4 represent the same experiments. In experiment #1 (A-B), daily clinical scores for mice treated with GMCSF-MOG were significantly different from those treated with "GM-CSF + MOG35-55" (days 12-42), MOG35-55 (days 14-42), GM-CSF (days 12-42), and saline (days 10-52). In experiment 2 (C-D), daily clinical scores for mice treated with GMCSF-MOG were significantly different from those treated with "GM-CSF + MOG35-55" (days 14-52), MOG35-55 (days 16-52), and saline (days 13-52).