Figure 9

GMCSF-MOG promoted tolerogenic activity despite ongoing inflammation in the CNS. Passive EAE was induced in C57BL/6 mice by adoptive transfer of activated MOG35-55-specific T cells on day 0 and by injection of Pertussis toxin on days 0 and 2. Mice were matched for EAE severity on day 9 (mean maximal severity of 0.8 and an incidence of 100% for all groups), and were then treated with GMCSF-MOG TTV or controls (subcutaneous in saline) on day 9 (4 nanomoles), day 11 (4 nanomoles), and day 14 (2 nanomoles). Mice were challenged with MOG35-55 in CFA on day 42 and Pertussis toxin was injected i.p. on days 42 and 44 to elicit a second bout of active EAE. Table 5 and Figure 9 represent the same experiment. Daily clinical scores for mice treated with GMCSF-MOG were significantly different from those treated with "MOG35-55" (days 54-57, 61-63, 65-70) or saline (days 28-58, 61-70). Thick arrows represent day 0 (adoptive transfer) and day 42 (active immunization). Thin arrows represent days that mice were treated with GMCSF-MOG, MOG35-55, or saline.