Figure 5

Comparison of levels of variable glycosylation between total IgG and affinity-purified autoantibodies. A: Comparison of levels of variable region glycosylation between total IgG and purified autoantibodies in ELISA. Group a: healthy controls. Open squares represent total IgG. Group b: patients with positive anti-MPO antibodies. Open squares represent total IgG; Filled squares represent affinity-purified anti-MPO antibodies. Group c: patients with positive anti-GBM antibodies. Open squares represent total IgG; Filled squares represent affinity-purified anti-α3(IV)NC1 antibodies. B: Comparison of levels of variable glycosylation between total IgG and purified autoantibodies by Western-blot analysis. The concentration of all samples was 1 μg/mL. Lane 1: mixture of affinity-purified anti-MPO antibodies from patient 11-16; lane 2: mixture of total IgG from patient 11-16; lane 3: mixture of affinity-purified anti-α3(IV)NC1 antibodies from patient 11-16. C: Correlation between the levels of variable glycosylation of anti-MPO antibodies and BVAS.