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Figure 1 | BMC Immunology

Figure 1

From: Increased levels of prolactin receptor expression correlate with the early onset of lupus symptoms and increased numbers of transitional-1 B cells after prolactin treatment

Figure 1

Purification of B cell subsets by flow cytometry. B cells were purified by negative selection from 9-week-old mice. Splenic cells were incubated with different antibodies (anti-CD19, anti-CD93, anti-CD21, anti-CD23, and anti-IgM), and the different B cell subsets were purified by flow cytometry as detailed in the Methods section. The purity of the collected populations oscillated between 95% and 98%. A representative example of the purified B cells from wild-type C57BL/6 mice is shown.

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BMC Immunology

ISSN: 1471-2172

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