Absolute numbers of mature B cells after the induction of hyperprolactinaemia. Nine-week-old mice were treated with metoclopramide (100 μg/100 μl) [hyperprolactinaemia (HyperPRL)] or with PBS (100 μl) for 6 weeks, with fourteen mice per condition in the C57BL/6 and MRL/lpr groups, and seven mice per condition in the MRL group. At the end of this treatment, splenocytes were marked with antibodies against CD19, CD93, CD21, IgM and CD23, and the absolute number of each population was calculated. A) The absolute number of splenic B cells. B) Representative dot plot of the mature MZ and FO B cell subsets. C) Graph of the absolute number of MZ B cells. D) Graph of the absolute number of FO B cells. The asterisks denote statistical significance between populations with the p value shown.