Signal transduction in Jurkat T cells. Jurkat T cells were stimulated with different combinations of stimuli in order to elucidate the different signal transduction pathways. A; Jurkat T cells were stimulated as indicated and intracellular Ca2+ release was monitored over/in time. B; Intracellular signal transduction routes were charted via phosphoanalysis using western blot. Jurkat T cells were stimulated for 15 min using different stimulations. Proximal (Lck, ZAP70, PKCθ and the PKC substrate MARCKs), medial (MAPK phosphorylation) and distal (c-Jun and ATF2) signaling was monitored based on the phosphorylation status of the described proteins. C; Nuclear translocation of the transcription factors NFAT, NFkB and c-Jun was evaluated 15 minutes after stimulation.