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Figure 1 | BMC Immunology

Figure 1

From: Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling

Figure 1

Signal transduction in Jurkat T cells. Jurkat T cells were stimulated with different combinations of stimuli in order to elucidate the different signal transduction pathways. A; Jurkat T cells were stimulated as indicated and intracellular Ca2+ release was monitored over/in time. B; Intracellular signal transduction routes were charted via phosphoanalysis using western blot. Jurkat T cells were stimulated for 15 min using different stimulations. Proximal (Lck, ZAP70, PKCθ and the PKC substrate MARCKs), medial (MAPK phosphorylation) and distal (c-Jun and ATF2) signaling was monitored based on the phosphorylation status of the described proteins. C; Nuclear translocation of the transcription factors NFAT, NFkB and c-Jun was evaluated 15 minutes after stimulation.

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BMC Immunology

ISSN: 1471-2172

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