PMA/CD3 and CD3/28 stimulations differ from PMA/CD28 stimulation. The table shows the number of regulated probe sets and their overlap at 1 and 8 hrs following stimulation with CD3/PMA, PMA/CD28 and CD3/CD28 (using a fold change cut off of 2 and a p-value cut off of < 1.10-6). The right panel shows a hierarchical clustering of the data using the data for the regulated probe sets. Most of the variation is caused by the time difference (1 hr vs 8 hrs). At both time points the PMA/CD28 stimulus was clearly different from the CD3/CD28 and CD3/PMA stimulus. Repeating this analysis with different values for the fold change and p-value cut off yielded essentially the same results for the multivariate analysis.