Figure 1

Identification of HLA class I and II restricted T cell epitopes from host proteins that shared similarity with M. tuberculosis antigens. Protein sequences of M. tuberculosis were subjected to BLAST search with the human proteome for identifying similarity regions. The peptides were analyzed for HLA binding for predominantly occurring HLA class I and II alleles using NetMHC server as described in methods. Peptides binding to HLA class I and class II molecules were considered to be CD8 T cell and CD4 T cell epitopes respectively. The peptides were classified based on predicted IC₅₀ value as strong binders (IC₅₀ ≤ 500), weak binders (500 ≤ IC₅₀ ≤ 5000) and non-binders (IC₅₀ ≥ 5000). The total number of binders (strong and weak) for each allele is represented as bar diagrams for (A) HLA class II and (B) HLA class I molecules.