Figure 3

Analysis of the role of HIV-1 Tat protein in the regulation of IP-10 expression. (A) Huh7.5.1 cells were infected with or without JFH1 virus and then incubated with PBS, 1 μg/ml Tat, or 1 μg/ml heat-inactivated Tat (HI-Tat) for different times, as indicated. Cell culture supernatants were collected and IP-10 levels were quantified by ELISA. (B) Huh7.5.1 cells were infected with or without JFH1 virus and then incubated with PBS, 1 μg/ml Tat, or 1 μg/ml heat-inactivated Tat (HI-Tat) for different times, as indicated. Cells were collected and the levels of IP-10 mRNA were quantified by real-time PCR (RT-PCR).