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Table 1 Primer sequences for PCR amplification of col7a1 cDNA fragments

From: Prevalence of collagen VII-specific autoantibodies in patients with autoimmune and inflammatory diseases

Fragment

Size (bp)

Primer sequences (5'-3')

NC1

2573

FP: GATCCTGGGCCCCACATCCATCCTC

  

RP: GATCGAATTCGCCCGGGAGGCCAGGGTCG

NC2

524

FP: GATCGAATTCGGCGAGAAGGGAGAAGCTGC

  

RP: GATCAAGCTTTCAGTCCTGGGCAGTACCTGT

  1. FP forward primer, RP reverse primer
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BMC Immunology

ISSN: 1471-2172

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