Figure 2

CD39⁺ Treg cells display strong suppressive effects and highly express the functional maker. (A) The percentage of CTLA-4, HLA-DR and Ki-67 expression on CD39⁺ or CD39^- Treg cells. Dot plots gated on CD4⁺ T cells are shown with representative results. (B) Proliferative capacities of CD4⁺CD25^- responder alone and with autologous FACS-sorted CD4⁺CD25⁺CD39⁺ Tregs or CD4⁺CD25⁺CD39^- Tregs subsets following anti CD3/anti-CD28 stimulation at the indicated S/R ratios on day 3. Statistical data are from three separate experiments with different donors. * P < 0.01, vs. the effects of CD39⁺ Tregs at the corresponding S/R ratios.