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Table 3 Relative expression of DE genes in SL samplesa from microarray vs. qRT-PCR analysisb

From: Understanding mechanisms of vitiligo development in Smyth line of chickens by transcriptomic microarray analysis of evolving autoimmune lesions

   NV EV AV CV
GeneSymbol Accession # Micro-array RT-PCR Micro-array RT-PCR Micro-array RT-PCR Micro-array RT-PCR
IRF1 L39766 1.58 1.73 5.72 7.32 5.22 8.08 2.52 3.46
TLR15 DQ267901 1.23 1.47 5.49 12.54 7.62 18.41 2.59 3.66
CR2 AJ720954 0.21 9.88 2.33 54.92 2.96 174.26 2.16 62.87
POU2AF1 AJ720333 0.96 3.63 2 18.32 3.89 61.51 4.07 67.04
B2M AB178593 0.98 1.28 1.77 3.46 2.94 4.82 1.66 3.01
CRYAB U26661 1.14 1.41 0.18 0.53 0.24 0.19 0.56 0.09
NPY M87294 1.03 0.79 0.13 0.49 0.41 0.1 0.94 0.004
TRAF5 AJ720372 0.37 1.44 1.4 4.61 2.09 6.39 1.24 3.99
LITAF AB058634 1.41 1.42 5.48 13.21 14.05 21.88 9.29 11.23
TYR AB023291 1.22 1.12 1 0.8 0.4 0.26 0.07 0.001
TRP1 AF003631 1.03 1.25 1.03 0.51 0.24 0.04 0.03 0
  1. a, SL samples included NV, EV, AV and CV samples which were from SL chickens that never developed vitiligo; from vitiliginous SL chickens within 1 week before SLV onset, during active depigmentation and at least one week after complete loss of pigmentation, respectively
  2. b, Gene expression in NV, EV, AV and CV was presented by fold changes relative to expression in BL samples in microarray and in qRT-PCR, where fold-change was determined by the delta delta Ct method. The same RNA pools for NV, EV, AV, CV and BL samples used in the microarray study were reverse transcribed to cDNA, which was subjected to qPCR with GAPDH as the endogenous control gene and cDNA from BL sample as the calibrator. All values are fold change means of three replications analyzed by JMP Genomics 4 for microarray and SYSTAT for qRT-PCR data