Figure 1

GMFG is enriched in lymphocyte pseudopodia. Panel A) The proteins in isolated pseudopodia (PS) or whole cell lysates (WC) of Jurkat T cells separated by SDS-PAGE. The levels and protein patterns were determined by staining for total protein (left panels) and the levels of GMFG in each fraction were assessed by western blot (right panels). Panel B) Quantitation of the intensity of the GMFG bands observed in the western blots in A. The GMFG levels were 45% greater in the pseudopodia than in the whole cell lysate (n = 3, * p = 0.017). Panels C-F) Confocal images of GMFG distribution in IL-2 dependent T cells. C, E) Arrowheads indicate the presence of GMFG at the leading edge of the cell. D, F) Difference interference contrast images of the same cells for comparison of cell morphology.