Figure 2
From: Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes
Effects of shRNA mediated reduction of GMFG expression on T cell migration. A) The efficiency of GMFG knockdown in Jurkat cells was assessed by western blot. Lanes 1–3 contained lysates from lines generated with three different shRNA targeting GMFG. Lanes NS and Jrkt respectively contained cells transduced with a non-silencing control vector or untreated Jurkat cells. B) The levels of GMFG expression (grey bars) and chemotactic responses (black bars) of each cell line were compared. Values are presented as a percentage of the wild type expression and migration levels (N = 3, +/− SEM).