Figure 3

The effects of GMFG knockdown on T cell movement. Panel A) Control IL-2 dependent T cells containing a non-silencing construct displayed random movement on a fibronectin coated surface (upper panel) which was not enhanced by the addition of SDF1 (middle panel). GMFG knockdown resulted in the abrogation of most of the detectable movement, even in the presence of SDF1 (lower panel). Panel B). Significant reductions were observed for the velocity (upper panel), accumulated distance (middle panel) and Euclidean distance (lower panel) of GMFG knockdown cells relative to control cells. The difference between non-silencing and GMFG knockdown groups was statistically significant ( p < 0.001) in all three parameters.