Figure 2

Schematic representation of the chimeric KIR-CD300a receptors. Structure of the chimeric molecules bearing the extracellular domain of KIR2DL2 and the transmembrane and cytoplasmic domains of CD300a is represented. The construct is fused to the hemagglutinin (HA) tag at the C-terminus. The shady dotted regions in the cytoplasmic tail correspond to the ITIMs in the CD300a receptor. The tyrosine in each of the 4 ITIMs was mutated to phenylalanine to generate the mutant chimeric construct KIR-CD300a 4F.