Figure 5
From: Functional requirements for inhibitory signal transmission by the immunomodulatory receptor CD300a
The phosphatases SHP-1 and SHP-2 associate with tyrosine phosphorylated CD300a ITIMs. KIR-CD300a Jurkat T cells were stimulated with medium or pervanadate for 3 minutes, or incubated for 5 minutes at 37°C with 721.221-Cw3 and 721.221-Cw6 cells. Then, cell lysates were immunopreciprecipitated with anti-KIR2DL2 (clone GL183) mAb and blotted separately for HA, SHP-1 and SHP-2. Results are representative of two independent experiments.