Figure 5
Influence of neuropeptides on ROS generation by human PMNs. Human PMNs (105/well) were added to ~107 CFU of nonopsonized or 10% heat-inactivated serum-opsonized M. catarrhalis ATCC 25238 (Mc) or nontypeable H. influenzae ATCC 49247 (NTHi) or to wells without bacteria in the presence or absence of 10−8 M of given neuropeptides and luminol-dependent chemiluminescence was measured up to 60 min using Berthold Microlumat luminometer. (A) Kinetics of ROS generation in human PMNs in response to bacterial stimulation in the absence of NPs. The results are expressed as mean relative light units/sec (RLU/s). The representative experiment performed in triplicate is shown (B) Histograms represent the AUCCL (mean ± SD) of at least 3 independent experiments done in triplicate from different donors. Results are expressed as a percentage of the PMN response stimulated only with bacteria. Asterisks indicate statistically significant differences calculated with reference to relevant controls if *p ≤ 0.05 and **p < 0.0005 (one-way ANOVA followed by Duncan’s post hock test).