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Table 3 Neuropeptide-mediated stimulation of fMLF-induced ROS generation by PMNs in luminol-dependent chemiluminescence

From: Innate immune properties of selected human neuropeptides against Moraxella catarrhalis and nontypeable Haemophilus influenzae

Stimulus

ROS release [% of control]

 

SP

NPY

CGRP

 

10-8 M

10-10 M

10-12 M

10-8 M

10-10 M

10-12 M

10-8 M

10-10 M

10-12 M

fMLF

179.18a

160.36a

140.83a

113.33

136.04a

129.67c

266.41a

164.35a

153.47b

 

(4.99)

(3.80)

(5.22)

(6.64)

(3.91)

(3.26)

(8.37)

(13.47)

(1.5)

  1. Human PMNs (105 cells/well) were incubated in the presence of 0.2 μM of fMLF and three concentrations of each NPs and chemiluminescence was measured up to 30 min using Berthold Microlumat luminometer. Values are expressed as relative changes from controls (PMNs + fMLF) and were calculated based on the maximal CL increase (PHCL). The presented results are means ± (SEM) of three independent experiments performed at least in triplicate. a-c Significantly different from fMLF-stimulated ROS increase (ap < 0.0005; bp < 0.001; cp < 0.05) as determined by Tuckey’s post hock test.
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BMC Immunology

ISSN: 1471-2172

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