Table 1 Primer used for Reverse Transcriptase (RT-PCR)-polymerase chain reaction of cytokines, death receptors, and apoptosis markers in HME patients and controls
Name | Primer sequence | Product size (bp) | GenBank Accession numbers |
|---|---|---|---|
TLR2 | F: 5'-GGA AGA ATC CTC CAA TCA GGC-3' | 100 | NM_003264 |
R: 5'-CTT CTG TGA GCC CTG AGG GA-3' | |||
TLR4 | F: 5'-AGC CAC GCA TTC ACA GGG–3', | 100 | NM_138554 |
R: 5'-CAT GGC TGG GAT CAG AGT CC-3' | |||
TLR-9 | F: 5’- GTG ACA GAT CCA AGG TGA AGT-3’ | 487 | NM_017442 |
R: 5’- CTT CCT CTA CAA ATG CAT CAC T-3’ | |||
Fas | F: 5-TGA AGG ACA TGG CTT AGA AGT G-3’ | 117 | NM_000043 |
R: 5-GGT GCA AGG GTC ACA GTG TT-3’ | |||
FasL | F: 5- GCA GCC CTT CAA TTA CCC AT-3’ | 100 | NM_000639 |
R: 5-CAG AGG TTG GAC AGG GAA GAA-3’ | |||
TNFR p55 | F: 5'-TGT GTC TCC TGT AGT AAA TG-3' | 589 | NM_001065 |
R: 5'-ACG AAT TCC TTC CAG CGC AA-3' | |||
GAPDH | F: 5-GAA GGT GAA GGT CGG AGT C-3’ | 225 | NM_002046 |
R: 5-GAA GAT GGT GAT GGG ATT TC-3’ |