Figure 1

(A): 12% SDS PAGE gel stained with Coomassie blue shows Stx2 preparation was pure as it contained only A and B subunits of Stx2 (lane 2); the molecular weight markers (in thousands) were in lane 1. (B): Survival of mice given intraperitoneally PBS or 30 μg of HuMAbs 5C12 or 5H8 followed 4 hours later with intravenous administration of 100 ng of ¹²⁵I-Stx2. Mice in PBS group died between 57–64 h or 2.3-2.6 days after ¹²⁵I-Stx2 injection. Both 5C12 and 5H8 protected 100% of mice until the end of the experiment (264 h or 11 days).