Analysis of inflammatory chemokines, CX3CL1 and TGF-β1 in wound tissues. Inflammatory chemokines, CX3CL1 and TGF-β1 were detected in non-wounded (Day 0) and wounded (4, 7, 10 and 13 days) skin of the same animals in the 3 groups of mice. (A-E) Representative RT-PCR results from three independent experiments with three animals per group are shown. (B) The ratios of RT-PCR signals for the indicated effectors to β-actin were calculated. Values represent the mean ± SEM. *P < 0.05, diabetic vs. control; +P < 0.05, diabetic + WP vs. control; #P < 0.05, diabetic + WP vs. diabetic (ANOVA with Tukey’s post-test).