Figure 4

Mitogen-stimulated proliferations of splenocytes. Splenocytes isolated from mice (8 animals/group) received twice subcutaneous injections at weeks 0 and 3 of split H1N1 influenza virus antigen (equivalent to 10 and 100 ng HA) or inactivated H1N1 influenza virus antigen (equivalent to 10 ng HA) co-administrated with docetaxel (25, 50, 100 and 200 μg) or alum (200 μg) and docetaxel administrated alone. Splenocytes were prepared 2 weeks after the last immunization and cultured with ConA, LPS or RPMI 1640. Splenocyte proliferation was measured by the MTT method as described in the text, and is shown as a stimulation index (SI). Values represent mean ± S.D. Significant differences with 10 ng HA groups were designated as *P < 0.05 and **P < 0.01.