Expression of cytokines and transcript factors mRNA by splenocytes. Mice (8 animals/group) received twice subcutaneous injections at week 0 and 3 of inactivated H1N1 influenza virus antigen (equivalent to 10 ng HA) alone or with docetaxel (100 μg) or alum (200 μg). Mice injected with saline only were used as a calibrator group. Splenocytes were prepared 2 weeks after the second immunization and cultured with HA (1.32 μg/ml) for 10 h. mRNA expression of cytokines IFN-γ, IL-12, IL-4 and IL-10 (A) and transcript factors T-bet and Gata-3 (B) was analyzed by real-time PCR. Results are reported as the n-fold difference relative to cytokine mRNA expression of calibrator sample. Significant differences with 10 ng HA groups were designated as *P < 0.05 and **P < 0.01.