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Table 1 Sequences of primer and probe for quantitative RT-PCR of cytokine and transcription factor

From: Adjuvant effect of docetaxel on the immune responses to influenza A H1N1 vaccine in mice

Gene Primer sequence
ß-Actin Forward: 5′-AGCGGTTCCGATGCCCT-3′
  Reverse: 5′-AGAGGTCTTTACGGATGTCAACG-3′
  Probe:5′HEX-TCCTTCTTGGGTATGGAATCCTGTGGC-BHQ-13′
IL-4 Forward: 5′-GAGACTCTTTCGGGCTTTTCG-3′
  Reverse: 5′-CAGGAAGTCTTTCAGTGATGTGG-3′
  Probe: 5′FAM-CCTGGATTCATCGATAAGCTGCACC-BHQ-1 3′
IL-10 Forward: 5′-CCAGTTTTACCTGGTAGAAGTGATG-3′
  Reverse: 5′-CTTGCTCTTATTTTCACAGGGGAG-3′
  Probe: 5′FAM-CAGGCAGAGAAGCATGGCCCAGAAA-BHQ-1 3′
IL-12p40 IL-12p40 Forward: 5′-TTGCTGGTGTCTCCACTCATG-3′
  Reverse: 5′-GTCACAGGTGAGGTTCACTGTTTC-3′
  Probe: 5′FAM-CTGGACTCCCGATGCCCCTGG-BHQ-1 3′
IFN-γ Forward: 5′-GCTTTGCAGCTCTTCCTCATG-3′
  Reverse: 5′-CTTCCACATCTATGCCACTTGAG-3′
  Probe: 5′FAM-CTGTTTCTGGCTGTTACTGCCACGGC-BHQ-1 3′
GATA-3 Forward: 5′-GGTCAAGGCAACCACGTC-3′
  Reverse: 5′-CATCCAGCCAGGGCAGAG-3′
  Probe: 5′FAM-CGCCCGCCTCTGCTGCACG-BHQ-1 3′
T-bet Forward: 5′-ATTGCCCGCGGGGTTG-3′
  Reverse: 5′-GACAGGAATGGGAACATTCGC-3′
  Probe: 5′FAM-CTGGGAAGCTGAGAGTCGCGCTCA-BHQ-1 3′