Table 1 Sequences of primer and probe for quantitative RT-PCR of cytokine and transcription factor
From: Adjuvant effect of docetaxel on the immune responses to influenza A H1N1 vaccine in mice
Gene | Primer sequence |
|---|---|
ß-Actin | Forward: 5′-AGCGGTTCCGATGCCCT-3′ |
|  | Reverse: 5′-AGAGGTCTTTACGGATGTCAACG-3′ |
|  | Probe:5′HEX-TCCTTCTTGGGTATGGAATCCTGTGGC-BHQ-13′ |
IL-4 | Forward: 5′-GAGACTCTTTCGGGCTTTTCG-3′ |
|  | Reverse: 5′-CAGGAAGTCTTTCAGTGATGTGG-3′ |
|  | Probe: 5′FAM-CCTGGATTCATCGATAAGCTGCACC-BHQ-1 3′ |
IL-10 | Forward: 5′-CCAGTTTTACCTGGTAGAAGTGATG-3′ |
|  | Reverse: 5′-CTTGCTCTTATTTTCACAGGGGAG-3′ |
|  | Probe: 5′FAM-CAGGCAGAGAAGCATGGCCCAGAAA-BHQ-1 3′ |
IL-12p40 | IL-12p40 Forward: 5′-TTGCTGGTGTCTCCACTCATG-3′ |
|  | Reverse: 5′-GTCACAGGTGAGGTTCACTGTTTC-3′ |
|  | Probe: 5′FAM-CTGGACTCCCGATGCCCCTGG-BHQ-1 3′ |
IFN-γ | Forward: 5′-GCTTTGCAGCTCTTCCTCATG-3′ |
|  | Reverse: 5′-CTTCCACATCTATGCCACTTGAG-3′ |
|  | Probe: 5′FAM-CTGTTTCTGGCTGTTACTGCCACGGC-BHQ-1 3′ |
GATA-3 | Forward: 5′-GGTCAAGGCAACCACGTC-3′ |
|  | Reverse: 5′-CATCCAGCCAGGGCAGAG-3′ |
|  | Probe: 5′FAM-CGCCCGCCTCTGCTGCACG-BHQ-1 3′ |
T-bet | Forward: 5′-ATTGCCCGCGGGGTTG-3′ |
|  | Reverse: 5′-GACAGGAATGGGAACATTCGC-3′ |
|  | Probe: 5′FAM-CTGGGAAGCTGAGAGTCGCGCTCA-BHQ-1 3′ |