Table 2 Sequences of primer for quantitative RT-PCR of microRNA
From: Adjuvant effect of docetaxel on the immune responses to influenza A H1N1 vaccine in mice
MicroRNAs | Primer sequence |
|---|---|
miR-181a | Forward: 5′-AACATTCAACGCTGTCGGTG-3′ |
miR-155 | Forward: 5′-GGGGTTAATGCTAATTGTGATAGG-3′ |
miR-150 | Forward: 5′-TCTCCCAACCCTTGTACCA-3′ |
miR-146a | Forward: 5′-GGGTGAGAACTGAATTCCATGG-3′ |
miR-125b | Forward: 5′-TCCCTGAGACCCTAACTTGTGA-3′ |
Universal reverse primer | Reverse primer: 5′-GCGAGCACAGAATTAATACGACTC-3′ |
Reverse transcription primer | Poly (T) adapter: 5′-GCGAGCACAGAATTAATACGACTCACTATAGG (T)12VN-3′ (V = A,G,C; N = A,T,G,C) [Virginie Olive 2009] |