γδ T cell–deficient mice have markedly impaired neutrophil recruitment during S. aureus -induced pneumonia. WT and TCR-δ−/− mice (3–6 mice/group) were intranasally inoculated with S.aureus. Cells were prepared from the lung at 6 h post infection and neutrophils were detected by flow cytometry. The representative dot-plots (A) and statistical results (B) were shown (n = 6). (C) Lungs of TCR-δ−/− mice and WT were lavaged and the number of neutrophils in BAL fluid was determined by flow cytometry. *p < 0.05 versus WT.