Figure 1

Clinical parameter data presented for individual wild-type mice on a daily time scale. Wild-type mice were treated with 3% (A, C, E) or 1% (B, D, F) DSS (n = 5–6 mice/group) in their drinking water for seven days, then supplied with regular drinking water for seven days (closed symbols). Control mice received normal drinking water throughout (open symbols on the X axis) Note that all 1% DSS mice scored 0 and overlap with the control mice on the X axis. Mice were scored for the presence of occult blood (A, B) and stool consistency (C, D) on a daily basis during the fourteen day experiment as described in the materials and methods. (E, F) Mice were weighed on a daily basis to calculate the percent change in weight for the treatment (days 0–7) and recovery (days 7–14) periods. One of two experiments (5–6 mice per condition) with similar results shown. *** p < 0.001; Student’s t-test; n.s., not significant.